Introduction
Evaluation
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Solution |
Tube no. |
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1
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2
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3
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4
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5
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6
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UNK
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0.35 mg/ml BSA
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-
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0.2
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0.4
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0.6
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0.8
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1.0
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-
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Unknown
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-
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-
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-
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-
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-
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-
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0.5
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Distilled water
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1.0
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0.8
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0.6
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0.4
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0.2
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-
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0.5
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Alkaline CuSO4
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5.0
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5.0
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5.0
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5.0
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5.0
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5.0
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5.0
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2- Mix well and leave at room temperature for 10 minutes |
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3-Dilute Folin reagent
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0.5
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0.5
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0.5
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0.5
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0.5
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0.5
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0.5
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4-Immediately mix and then leave at room temperature for 30 minutes |